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The culture conditions and cell composition of a hydrogen bacterica, Alcaligenes eutrophus ATCC 17697. were investigated. Optimum pH and temperature for cell growth under autotrophic condition (H₂ as energy source, CO₂, as crabon source) were around 7.0 and 30℃. respectively. Effect of oxygen partial pressure in the range of 0.059 atm and 0.27 atm on cell growth was also studied. Maximum specific growth rate (μ_(max)=0.31hr^(-1)) was observed at 0.11 atm of oxygen partial pressure (H₂:O₂:CO₂=7:1:1). The contents of crude protein, nucleic acid and ash in cells were 69.2%, 17.6%, and 3.62%, respectively.
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